By A. Zlatkis and R.E. Kaiser (Eds.)
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Additional resources for HPTLC High Performance Thin-Layer Chromatography
In this case it is most important that the chamber is tightly closed. 5 mm. The best utilization of the indium plates is probably first possible in a Uchamber with automaticallycontrolled,variable developing solventtravelling speeds. , with a normal teflon tube, inner diameter of ca. 6 mm. , in the search for an optimal solvent system) or application of more solutions, whose components are to be compared singly and against each other. In the simplest case, where only one substance is to be applied, when the solvent and solvent system are the same and the sample volume is small in comparison to the wick volume, then the sample can be applied directly onto the dry wick.
G. g. viscosity should have low values. 03 is a difficult requirement. Is there any practical value for such high separation numbers? It is usually emphasized that the efficiency in chromatography of the liquid phase is achieved rather by a good selectivity than by high separation numbers. According to L. Rohrschneider (Innsbruck, May 1975) this is not true. Selectivity is aimed at separating two substances, and can be optimized for these two substances. However optimization means consideration of the mobile phase and this can be a very tedious process.
Dimensions: in mm, which is the same scale as the time values or the equivalent migration distances. = Imporrant: The time values are recorded by fast, linear scanning of the TLC chromatogram along the migration distance. It is obvious from the above figure that the t/b ratio is dependent on the t value. This is the precise point: since the theoreticians are not concerned with the practical aspect that each chromatographic analysis requires time and material, they neglected a very simple fact, which will be presented in the following figure.
HPTLC High Performance Thin-Layer Chromatography by A. Zlatkis and R.E. Kaiser (Eds.)